Panadea ZIKV IgG ELISA Kit

The Panadea ZIKV IgG ELISA Kit is intended for qualitative detection of IgG antibodiesdirected
against the Zika Virus (ZIKV)

The Panadea ZIKV IgG ELISA Kit is based on the patented IgG FcγR ELISA technology. Samples are co-incubated togetherwith a horseradish peroxidase (HRP)-labeled recombinant ZIKV antigen and unlabeled protein competitors, suppressingcross-reactivity of other flaviviral infections in a microwell plate coated with a recombinant IgG immune complex specificcapture molecule.

During the incubation, IgG antibodies in the sample, the ZIKV antigens, and the competitor molecules form immunecomplexes, which then bind specifically and with high affinity to the capture molecules. All antibodies not binding to theantigen or the competitors, as well as any excess labeled antigen and competitors, are removed in the subsequentwashing step.

The bound IgG/antigen immune complexes are visualized by application of the colorimetric HRP substrate tetramethylben-zidine (TMB). After stopping the enzymatic reaction, the assay result is generated by measuring the optical density (OD) ofthe solution in the well at 450/620 nm.

PRODUCTINFORMATION.
REF
ELG.003
Size.
96-wells
Principle
IgG FcγR ELISA
Type
Qualitative
SampleType
Serum
Antigen
Envelope protein domain III.
Storage
Store Conjugate at -20°CStore all other kit components at 2°C - 8°C.
KIT CONTENTS
Microwell Plate (IgG)
1piece,12strips
Positive Control
1 vial
Negative Control
1 vial
Cut-off Control
1 vial
Sample Dilution Buffer (SDB)
1 bottle
ConjugateDilutionBuffer(CDB)
1 bottle
10X Wash Buffer
1 bottle
Competitor Reagent
4 vials
Conjugate
1 vial
Substrate
1 bottle
Stop Solution
1 bottle
Adhesiev foil
2 pieces
Instruction for Use
1 piece

Anzinger et al. (2022), Antenatal Seroprevalence of Zika and Chikungunya Viruses, Kingston Metropolitan Area, Jamaica,2017-2019. Emerg Infect Dis. 28(2):473-475

Ehmen et al. (2021), Accurate detection of Zika virus IgG using a novel immune complex binding ELISA.Trop Med Int Health 26(1):89-101