Surveillance of Crimean-Congo Hemorrhagic Fever Virus prevalence through screening of population samples for antibodies against the virus has importance not only for populations where the disease is known to circulate, but also for public health system preparedness on a world-wide level. As CCHFV can spread from person to person via contact of blood and other bodily fluids, the detection of the CCHF virus in a population is important for the prevention of outbreaks.

The Panadea CCHFV IgG ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgG antibodies directed against the Crimean-Congo-Hemorrhagic Fever Virus (CCHFV) nucleocapsid protein (NP) in 96-well (12x8) format. The test is based on a patented immune complex capture principle.

References:
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- Emmerich P, von Possel R, Deschermeier C, Ahmeti S, Berisha L, Halili B, Jakupi X, Sherifi K, Messing C, Borchardt-Lohölter V. Comparison of diagnostic performances of ten different immunoassays detecting anti-CCHFV IgM and IgG antibodies from acute to subsided phases of Crimean-Congo hemorrhagic fever. PLoS Negl Trop Dis. 2021 Mar 15;15(3)
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- Lombe BP, Miyamoto H,Saito T, Yoshida R, Manzoor R, Kajihara M, Shimojima M, Fukushi S, Morikawa S, Yoshikawa T, Kurosu T, Saijo M, Tang Q, Masumu J, Hawman D, Feldmann H, Takada A. Purification of Crimean-Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis. SciRep. 2021 Jan 27;11(1):2324

- Rahden P, Adam A, Mika A,Jassoy C. Elevated Human Crimean-Congo Hemorrhagic Fever Virus Seroprevalence in Khashm el Girba, Eastern Sudan. Am J Trop Med Hyg. 2019Jun;100(6):1549-1551
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- Emmerich P, Mika A, von Possel R, Rackow A, Liu Y, Schmitz H, Günther S, Sherifi K, Halili B, Jakupi X,Berisha L, Ahmeti S, Deschermeier C. Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests. PLoSNegl Trop Dis. 2018 Mar 26;12(3)
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IgG antibodies persist in the blood of infected individuals long after the person has recovered from the viral infection. Observing the prevalence of a virus in a population, especially in Low- and Middle-Income Countries (LMIC), is most

The Panadea CCHFV IgM ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgM antibodies directed against the Crimean-Congo-Hemorrhagic Fever Virus (CCHFV) nucleocapsid protein (NP) in 96-well (12 x 8) format. The test is based on the µ-capture assay principle.

References:

- Emmerich P, von Possel R, Deschermeier C, Ahmeti S, Berisha L, Halili B, Jakupi X, Sherifi K, Messing C, Borchardt-Lohölter V. Comparison of diagnostic performances of ten different immunoassays detecting anti-CCHFV IgM and IgG antibodies from acute to subsided phases of Crimean-Congo hemorrhagic fever. PLoS Negl Trop Dis. 2021 Mar 15;15(3)

- Rackow A, Ehmen C, von Possel R, Medialdea-Carrera R, Brown D, Bispo de Filippis AM, Carvalho de Sequeira P, Ribeiro Nogueira RM, Halili B, Jakupi X, Berisha L, Ahmeti S, Sherifi K, Schmidt-Chanasit J, Schmitz H, Mika A, Emmerich P, Deschermeier C. Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies. Clin Chem. 2019 Mar;65(3):451-461

- Emmerich P, Mika A, von Possel R, Rackow A, Liu Y, Schmitz H, Günther S, Sherifi K, Halili B, Jakupi X, Berisha L, Ahmeti S, Deschermeier C. Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests. PLoS Negl Trop Dis. 2018 Mar 26;12(3)
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The Panadea ZIKV IgG ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgG antibodies directed against the Zika Virus (ZIKV) envelope protein domain III (EDIII) in 96-well (12 x 8) format. The test is based on a patented immune complex capture principle. Cross-reactivity with antibodies elicited by other flavivirus infections is suppressed by a specific mix of competitor molecules.

References:

- Anzinger JJ, Mears CD, Ades AE, Francis K, Phillips Y, Leys YE, Spyer MJ, Brown D, de Filippis AMB, Nastouli E, Byrne T, Bailey H, Palmer P, Bryan L, Webster-Kerr K, Giaquinto C, Thorne C, Christie CDC; ZIKAction Consortium1,2. Antenatal Seroprevalence of Zika and Chikungunya Viruses, Kingston Metropolitan Area, Jamaica, 2017-2019. Emerg Infect Dis. 2022 Feb;28(2):473-475

- Ehmen C, Medialdea-Carrera R, Brown D, de Filippis AMB, de Sequeira PC, Nogueira RMR, Brasil P, Calvet GA, Blessmann J, Mallmann AM, Sievertsen J, Rackow A, Schmidt-Chanasit J, Emmerich P, Schmitz J, Deschermeier C, Mika A. Accurate detection of Zika virus IgG using a novel immune complex binding ELISA. Trop Med Int Health. 2021 Jan;26(1):89-101

The Panadea ZIKV IgM ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgM antibodies directed against the Zika Virus (ZIKV) nonstructural protein 1 (NS1) in 96-well (12 x 8) format. The test is based on a patented immune complex capture principle.

References:
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- Rackow A, Ehmen C, von Possel R, Medialdea-Carrera R, Brown D, Bispo de Filippis AM, Carvalho de Sequeira P, Ribeiro Nogueira RM, Halili B, Jakupi X, Berisha L, Ahmeti S, Sherifi K, Schmidt-Chanasit J, Schmitz H, Mika A, Emmerich P, Deschermeier C. Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies. Clin Chem. 2019 Mar;65(3):451-461
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The Panadea LASV IgG ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgG antibodies directed against the Lassa Virus (LASV) nucleocapsid protein (NP) in 96-well (12 x 8) format. The test is based on a patented immune complex capture principle.

References:

- Longet S, Leggio C, Bore JA, Key S, Tipton T, Hall Y, Koundouno FR, Bower H, Bhattacharyya T, Magassouba N, Günther S, Henao-Restrapo AM, Rossman JS, Konde MK, Fornace K, Carroll MW. Influence of Landscape Patterns on Exposure to Lassa Fever Virus, Guinea. Emerg Infect Dis. 2023 Feb;29(2):304-313

- Soubrier H, Bangura U, Hoffmann C, Olayemi A, Adesina AS, Günther S, Oestereich L, Fichet-Calvet E. Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay. Viruses. 2022 May 7;14(5):993

- Gabriel M, Adomeh DI, Ehimuan J, Oyakhilome J, Omomoh EO, Ighodalo Y, Olokor T, Bonney K, Pahlmann M, Emmerich P, Lelke M, Brunotte L, Ölschläger S, Thomé-Bolduan C, Becker-Ziaja B, Busch C, Odia I, Ogbaini-Emovon E, Okokhere PO, Okogbenin SA, Akpede GO, Schmitz H, Asogun DA, Günther S. Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G. PLoS Negl Trop Dis. 2018 Mar 29;12(3):e0006361
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The Panadea LASV IgM ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgM antibodies directed against the Lassa Virus (LASV) nucleocapsid protein (NP) in 96-well (12 x 8) format. The test is based on the µ-capture assay principle.

References:

- Gabriel M, Adomeh DI, Ehimuan J, Oyakhilome J, Omomoh EO, Ighodalo Y, Olokor T, Bonney K, Pahlmann M, Emmerich P, Lelke M, Brunotte L, Ölschläger S, Thomé-Bolduan C, Becker-Ziaja B, Busch C, Odia I, Ogbaini-Emovon E, Okokhere PO, Okogbenin SA, Akpede GO, Schmitz H, Asogun DA, Günther S. Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G. PLoS Negl Trop Dis. 2018 Mar 29;12(3):e0006361

The Panadea SARS-CoV-2 IgG ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgG antibodies directed against the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) nucleocapsid protein (NP) in 96-well (12 x 8) format. The test is based on a patented immune complex capture principle.

References:

- Deschermeier C, Ehmen C, von Possel R, Murawski C, Rushton B, Amuasi J, Sarpong N, Maiga-Ascofaré O, Rakotozandrindrainy R, Asogun D, Ighodalo Y, Oestereich L, Duraffour S, Pahlmann M, Emmerich P. Fcγ-Receptor-Based Enzyme-Linked Immunosorbent Assays for Sensitive, Specific, and Persistent Detection of Anti-SARS-CoV-2 Nucleocapsid Protein IgG Antibodies in Human Sera. J Clin Microbiol. 2022 Jun 15;60(6):e0007522

The Panadea SARS-CoV-2 IgG High Sensitivity ELISA Kit (Research Use Only) allows the qualitative detection of human serum/plasma IgG antibodies directed against the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) nucleocapsid protein (NP) in 96-well (12 x 8) format. The test is based on a patented immune complex capture principle and has been optimised for detection of low levels of anti-SARS-CoV-2 IgG antibodies.

References:

- Struck NS, Lorenz E, Deschermeier C, Eibach D, Kettenbeil J, Loag W, Brieger SA, Ginsbach AM, Obirikorang C, Maiga-Ascofare O, Sarkodie YA, Boham EEA, Adu EA, Asare G, Amoako-Adusei A, Yawson A, Boakye AO, Deke J, Almoustapha NS, Adu-Amoah L, Duah IK, Ouedraogo TA, Boudo V, Rushton B, Ehmen C, Fusco D, Gunga L, Benke D, Höppner Y, Rasolojaona ZT, Rasamoelina T, Rakotoarivelo RA, Rakotozandrindrainy R, Coulibaly B, Sié A, Awuah AA, Amuasi JH, Souares A, May J. High seroprevalence of SARS-CoV-2 in Burkina-Faso, Ghana and Madagascar in 2021: a population-based study. BMC Public Health. 2022 Sep 5;22(1):1676

- Deschermeier C, Ehmen C, von Possel R, Murawski C, Rushton B, Amuasi J, Sarpong N, Maiga-Ascofaré O, Rakotozandrindrainy R, Asogun D, Ighodalo Y, Oestereich L, Duraffour S, Pahlmann M, Emmerich P. Fcγ-Receptor-Based Enzyme-Linked Immunosorbent Assays for Sensitive, Specific, and Persistent Detection of Anti-SARS-CoV-2 Nucleocapsid Protein IgG Antibodies in Human Sera. J Clin Microbiol. 2022 Jun 15;60(6):e0007522

- Lorenz E, Souares A, Amuasi J, Loag W, Deschermeier C, Fusco D, Benke D, A Rakotoarivelo R, Rasamoelina MT, Rakotozandrindrainy R, Sie A, Afum-Adjei Awuah A, May J, Eibach D, Struck N. Seroprevalence of SARS-CoV-2 in urban settings in three sub-Saharan African countries (SeroCoV): a study protocol for a household-based cross-sectional prevalence study using two-stage cluster sampling. BMJ Open. 2021 Dec 17;11(12):e056853